Calculate cells needed, volume to seed, and prepare cell suspensions for well plates, flasks, and dishes. Perfect for cell culture planning and experimental setup.
Fill in the form and click “Calculate” to see your cell seeding calculations.
Cell seeding density refers to the number of cells plated per unit area of the culture surface, typically expressed as cells per square centimeter (cells/cm²). This parameter is critical for reproducible experiments and affects cell behavior, growth kinetics, and experimental outcomes.
Cells/Well = Seeding Density (cells/cm²) × Surface Area (cm²)Viable Conc. = Stock Concentration × (Viability% / 100)Volume/Well = Cells/Well ÷ Viable ConcentrationTotal Volume = (Volume/Well × Number of Wells) × (1 + Overage%)| Vessel Type | Surface Area (cm²) | Typical Volume |
|---|---|---|
| 6-well plate | 9.5 | 2-3 mL |
| 12-well plate | 3.8 | 1-2 mL |
| 24-well plate | 1.9 | 0.5-1 mL |
| 96-well plate | 0.32 | 100-200 µL |
| T25 flask | 25 | 5-7 mL |
| T75 flask | 75 | 15-20 mL |
| 100mm dish | 55 | 10-12 mL |
Different cell types have different optimal seeding densities depending on their size, growth rate, and experimental requirements:
Cell viability assessment (typically using trypan blue exclusion) ensures you seed the correct number of viable cells. Non-viable cells in your suspension will not attach or proliferate, so the calculator adjusts for viability to ensure your target seeding density reflects only living cells.
For example, if your stock is 1×10⁶ cells/mL with 90% viability, your viable concentration is effectively 9×10⁵ cells/mL. The calculator uses this adjusted value to determine the correct volume to seed.
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