Estimate cell doubling time from two time points and cell counts, or simulate a simple exponential growth curve from a known doubling time and seeding density.
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Cell doubling time (Td) is the time required for a cell population to double in number under specific growth conditions. It's a fundamental metric in cell biology that characterizes the proliferation rate of cultured cells.
Typical doubling times vary widely: cancer cell lines may double in 18-24 hours, while primary cells often have longer doubling times of 48-72 hours or more.
During the log phase of growth, cells proliferate exponentially following the formula:
Where:
This is equivalent to the natural exponential form: N(t) = N₀ × e(k×t), where k = ln(2)/Td is the specific growth rate.
When you have cell counts at two time points, you can estimate doubling time using:
This calculation assumes cells were in exponential growth phase throughout the measurement period, with no significant lag or stationary phase effects.
Real cell cultures don't grow exponentially forever. Growth typically follows these phases:
This tool is for research and educational purposes only. It provides theoretical estimates based on simple exponential growth models. Actual cell behavior depends on numerous factors including cell type, passage number, media composition, seeding density, and culture conditions. Always follow your institution's protocols and manufacturer guidelines for cell culture experiments.
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